ABSTRACT
The establishment of human malignant tumor cell lines can provide abundant experimental materials for understanding the biological characteristics of tumors, studying the carcinogenesis, molecular genetics and the mechanism of metastasis and evolution. In this study, a novel cell line designated ZJB-ENC1 has been established from poorly differentiated endometrioid adenocarcinoma. Cytological results showed monolayer-cultured cells were polygonal in shape and a piling-up tendency without contact inhabitation. Immunohistochemistry analysis showed that the cells were negative for ER, PR, c-erbB2, E-CAD, CD117, and OCT3/4, but strongly positive for PTEN and P16. Meanwhile, the tumorigenicity of ZJB-ENC1 was confirmed by subcutaneous transplantation of the cells into a xenograft mouse model. In addition, the results of the whole exome sequencing revealed a unique genomic characteristic of ZJB-ENC1 cells, all common and novel SNPs and InDels were identified. In conclusion, this new stable cell line may promote basic and clinical research on endometrial cancer (EC).
ABSTRACT
Functional scaffolds that could mimic cells' natural growth state in vitro are crucial for meeting the requirements of complex biological systems. However, the compact macrostructure and poor cell survival macro-environment of biomaterials are still two major challenges limiting their practical applications in bone tissue regeneration. In this study, we fabricated a highly fluffy and porous biomineral hydroxylapatite (HA) encapsulated poly-l-lactic acid (PLLA) composite fibrous scaffold (fluffy-HAFSs) by employing an improved electrospinning technique combined with a bio-mineralization technique. In the scaffold, deep interconnected pores of 65 ± 35 µm formed among these fluffy HA fibers, which permitted the easy entry of cells into the fluffy-HAFSs with no extra help to achieve complicated 3D cell culture methodologies. Human mesenchymal stem cells (hMSCs) were seeded onto the composite fibrous scaffolds and cultured for 14 days in vitro. The morphology and biochemical activities of hMSCs were tested over the culture period. Evidence was provided for the hMSCs entry into the interior of the fluffy-HAFSs and achievement of 3D cell distribution. Furthermore, these hMSCs exhibited higher degrees of growth, osteogenic differentiation and mineralization than those on HA deposited traditional electrospun fibrous meshes (HAFMs). These results indicated that the novel fluffy-HAFSs might be potentially applied as bone repairing and regeneration scaffolds.
ABSTRACT
OBJECTIVE: Little is known about using glycated haemoglobin A1c (HbA1c) to diagnose diabetes in Chinese subjects over 50 years old. This study aims to evaluate HbA1c in diagnosing diabetes and identify the optimal threshold to be used in Chinese community subjects aged over 50 years. METHODS: A community-based cross-sectional survey was conducted from October 2010 to January 2011 in Shipai community of Guangzhou, Guangdong, China. A total of 1494 subjects (72·8%) aged over 50 years were investigated. Fasting plasma glucose (FPG1st ) and HbA1c were assayed in each participant. Diabetic candidates with FPG1st ≥ 5·6 mmol/l or HbA1c ≥ 39 mmol/mol (5·7%) were informed to undergo a 75-g oral glucose tolerance test (OGTT). Diagnosis of diabetes was made by 1999 World Health Organization criteria. Sensitivity and specificity of HbA1 c for diagnosing diabetes were calculated by receiver operating characteristics (ROC) curve. RESULTS: Among 1494 subjects, 161 subjects (10·8%) with previously diagnosed diabetes and 21 with missing data were excluded. Among the remaining 1312 subjects (87·8%), 861 subjects (65·6%) with either FPG1st ≥ 5·6 mmol/l or HbA1c ≥ 39 mmol/mol (5·7%) were invited to perform OGTT. Finally, 453 subjects (52·6%) performed OGTT (FPG2nd and 2-h plasma glucose were measured) and 54 subjects (11·9%) were identified as being diabetes. The area under ROC curve was 0·916 (0·887-0·940) for HbA1c and 0·972 (0·953-0·985) for FPG2nd in diagnosing diabetes (P = 0·045). An HbA1c threshold of 48 mmol/mol (6·5%) yielded the highest combination of sensitivity (75·9%) and specificity (95·5%) for diagnosing diabetes. CONCLUSION: An HbA1 c threshold of 48 mmol/mol (6·5%) was highly specific and had a good sensitivity for diagnosing diabetes among Chinese subjects aged over 50 years with FPG ≥ 5·6 mmol/l or HbA1c ≥ 39 mmol/mol (5·7%). This threshold may be suitable for diagnosing diabetes in Chinese subjects over 50 years old.
